Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194345_dfay CAAAGCTGCAGGCTGGCATGGTCCCCACCAGG(+CAGTGT)GATCCTCTGGCCCATCCACGCTGTGCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605194343_qo2l GGCCGACCCTGCACAGCGTGGATGGGCCAGAG(+CAGTGT)GATCCCTGGTGGGGACCATGCCAGCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605194341_s912 GGTGTCCCATGCTCACTTCAGCACTGTTCAGG(+CAGTGT)GTACCTGACGGGGCAGTCCCACCCTCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605194340_1fw2 TGTTGGAACAGAGGGTGGGACTGCCCCGTCAG(+CAGTGT)GTACCCTGAACAGTGCTGAAGTGAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605194338_02qq CCTGCCATTGTTGGTTTGATGAACACGATTAG(+CAGTGT)GTACCCAGTGTGATCCCACCCTCACCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605194337_m99m GGTGAGGTGGGGTGAGGGTGGGATCACACTGG(+CAGTGT)GTACCTAATCGTGTTCATCAAACCAACAATG NGG 10 8 17 8 24 0 100 5 10
    240605194334_vzgk TGAGTCTGTCCTCCTCCAAGGTCAGCCCTCGG(+CAGTGT)GATCCCAAAATAGTCCCTTCTGAAGGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605194333_5me2 GTCAGCACCCCCTTCAGAAGGGACTATTTTGG(+CAGTGT)GATCCCGAGGGCTGACCTTGGAGGAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240605194330_mzmw CCCTGAGGCACGAGGGGACTGTGTTCTCAGGG(+CAGTGT)GATCCACCAGGATAGTGCCTGACTACAGATC NGG 10 8 17 8 24 0 100 5 10
    240605194328_09uu ATCCTGATCTGTAGTCAGGCACTATCCTGGTG(+CAGTGT)GATCCCCTGAGAACACAGTCCCCTCGTGCCT NGG 10 8 17 8 24 0 100 5 10