Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194241_7shv GAAAGGACAGGGTGTTCAGCACGTGTAGTTGG(+CAGTGT)GTACCTGGCCTAGAGGATACCCCTGAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605194239_u3c9 CTAAGAAGCTTCAGGGGTATCCTCTAGGCCAG(+CAGTGT)GTACCCAACTACACGTGCTGAACACCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605194238_n4b6 GTAGGCCTGGCTAGCTAGCCAGTTTGCACTGG(+CAGTGT)GTACCCAGACTGGAATGGCAGGTGAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240605194236_a3nr AACCAGGTGGCTCACCTGCCATTCCAGTCTGG(+CAGTGT)GTACCCAGTGCAAACTGGCTAGCTAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605194234_utty GCTGGCAGGCCCTGAAGCACAAGTTCAGGCCG(+CAGTGT)GATCCGGGCATCAGGACTTCGTGTTCATACA NGG 10 8 17 8 24 0 100 5 10
    240605194232_j02b GTCCATGTATGAACACGAAGTCCTGATGCCCG(+CAGTGT)GATCCGGCCTGAACTTGTGCTTCAGGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605194230_libb CATGACAACAGTGGTGTCCACTCCCACATTCG(+CAGTGT)GATCCCAGGGTCCTACGTCCTCCTAAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605194227_33nt CCCGACTGCTTTAGGAGGACGTAGGACCCTGG(+CAGTGT)GATCCGAATGTGGGAGTGGACACCACTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605194226_1a0m ATCGGCTGATGAGACCGGGTTCCATCTAAGCG(+CAGTGT)GTACCTGACTAACTCTGGCTCACTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605194225_nfmm GGGAACAGCCCTAGTGAGCCAGAGTTAGTCAG(+CAGTGT)GTACCGCTTAGATGGAACCCGGTCTCATCAG NGG 10 8 17 8 24 0 100 5 10