Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193121_cf9b AATTGATTTTTACTCTTTACTATCCAAGTTTG(+CAGTGT)GATCCATTCAGAAGAGCTGGTTTTAAATTCT NGG 10 8 17 8 24 0 100 5 10
    240605193119_p6fg CGAATAGAATTTAAAACCAGCTCTTCTGAATG(+CAGTGT)GATCCAAACTTGGATAGTAAAGAGTAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605193118_9165 AAGTGCACCTTTGTTATATACTTATTATATTG(+CAGTGT)GTACCAAATACAGAAGAAAACTATAGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605193117_m20i TATCACAGAACTATAGTTTTCTTCTGTATTTG(+CAGTGT)GTACCAATATAATAAGTATATAACAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605193115_7578 GCAAGCAATTGCAGCACCTTCAGAATGTGGAG(+CAGTGT)GTACCGGGCTAGACCTAGCAAACAATGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605193113_xfnh GCTTGAGGACATTGTTTGCTAGGTCTAGCCCG(+CAGTGT)GTACCTCCACATTCTGAAGGTGCTGCAATTG NGG 10 8 17 8 24 0 100 5 10
    240605193111_7m8p AAGTGAACCCCGCTGTGATGTGAAAATTTCAG(+CAGTGT)GATCCACACCAATGAACAGAATAAGCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605193110_qqp9 GATCTTCTAGGCTTATTCTGTTCATTGGTGTG(+CAGTGT)GATCCTGAAATTTTCACATCACAGCGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605193107_vsfz ACTGTCATATTTATACATGGCTTTTCCCCTAG(+CAGTGT)GATCCTCAATCCTTCAGACCTAAACACAATT NGG 10 8 17 8 24 0 100 5 10
    240605193105_tshz CCTGAAATTGTGTTTAGGTCTGAAGGATTGAG(+CAGTGT)GATCCTAGGGGAAAAGCCATGTATAAATATG NGG 10 8 17 8 24 0 100 5 10