Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193015_n8ok TCTCCTCCCCCTCGACCCTCCTAAATAACACG(+CAGTGT)GTACCAGGCTTGCTGGCTGTGGGATTTACAG NGG 10 8 17 8 24 0 100 5 10
    240605193013_fb0n TATACCTGTAAATCCCACAGCCAGCAAGCCTG(+CAGTGT)GTACCGTGTTATTTAGGAGGGTCGAGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605193010_vthp TTGGTGGGAAGTCTGCTGGAAGAGGCTTCCGG(+CAGTGT)GTACCATTTAGGGGAAGTCAAACTTGTGACT NGG 10 8 17 8 24 0 100 5 10
    240605193008_qpu3 TTTATAGTCACAAGTTTGACTTCCCCTAAATG(+CAGTGT)GTACCCGGAAGCCTCTTCCAGCAGACTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605193006_gkzq AGGAATCTGGAACATGGGACCAAGGGTAAGGG(+CAGTGT)GATCCTTCAGGGATTTCAGGGAGAGCATGTT NGG 10 8 17 8 24 0 100 5 10
    240605193005_4qhk GGGGAAACATGCTCTCCCTGAAATCCCTGAAG(+CAGTGT)GATCCCCTTACCCTTGGTCCCATGTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605193003_vwa4 GCACAAAAAGAGTGTCGCTCAAGTTTAGTGGG(+CAGTGT)GTACCACACAAGGAATGAACACATTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605193002_pskf CTCATAGCCTCAATGTGTTCATTCCTTGTGTG(+CAGTGT)GTACCCCACTAAACTTGAGCGACACTCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605193001_gdr9 CATTTCCCTTCTGGGTACAAGGGCCTGTTTTG(+CAGTGT)GATCCTAGAATATCAAATATATACCACACCA NGG 10 8 17 8 24 0 100 5 10
    240605192959_m6s3 GAGTATGGTGTGGTATATATTTGATATTCTAG(+CAGTGT)GATCCAAAACAGGCCCTTGTACCCAGAAGGG NGG 10 8 17 8 24 0 100 5 10