Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192934_ypm9 CAGGGAATGAGATTGGCCCTTTCTCTGCCAGG(+CAGTGT)GTACCACAGGGACACATGCCGGGTAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605192932_wh4n CATACTCAGGCTACCCGGCATGTGTCCCTGTG(+CAGTGT)GTACCCTGGCAGAGAAAGGGCCAATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605192930_y9e0 TTGGTGAGGGTCCTGAGGTGCTCTGGCCACAG(+CAGTGT)GTACCATCCATAGCTCCAACTCATGTGACCA NGG 10 8 17 8 24 0 100 5 10
    240605192929_ont5 CCCTTTGGTCACATGAGTTGGAGCTATGGATG(+CAGTGT)GTACCTGTGGCCAGAGCACCTCAGGACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605192927_wloh TTAGTTAAAGCGACTGCTATGGGAGGCTTAGG(+CAGTGT)GATCCTCCTTGGAGGTTGGCCCCCTGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605192926_v4ex ACATCCTCAGCAGGGGGCCAACCTCCAAGGAG(+CAGTGT)GATCCCTAAGCCTCCCATAGCAGTCGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240605192924_lj53 GCCCACGGAGGGCCTTTATTCATTGTCCACAG(+CAGTGT)GTACCCTGGAGCTGTGGCCTCCAGGATAGAG NGG 10 8 17 8 24 0 100 5 10
    240605192922_ez0t GGTATCTCTATCCTGGAGGCCACAGCTCCAGG(+CAGTGT)GTACCTGTGGACAATGAATAAAGGCCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240605192920_9spz ATTCACCTGCTCTTTGGGCAAACATTCATTGG(+CAGTGT)GTACCACAACACCAAACCTCCCGAGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605192919_u4it TAGTTGCCTGTCTCGGGAGGTTTGGTGTTGTG(+CAGTGT)GTACCCAATGAATGTTTGCCCAAAGAGCAGG NGG 10 8 17 8 24 0 100 5 10