Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192836_6dh8 GCTTGTTTTCTCTTTGTAAAGGACAAACTATG(+CAGTGT)GTACCAGAATCTGCCAAAGATCCCCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605192835_f62o TGAGATGAGAAGGGGATCTTTGGCAGATTCTG(+CAGTGT)GTACCATAGTTTGTCCTTTACAAAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605192832_8pzh CTTGCTTAGAGCCAGAAGGGATGATGTCCTGG(+CAGTGT)GATCCAAGACCAGAGGGACAGCATCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605192831_1771 CTCCCCTCAGTGATGCTGTCCCTCTGGTCTTG(+CAGTGT)GATCCCAGGACATCATCCCTTCTGGCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240605192829_m8ps GTACATGAGTGTGGAAGTCTGAGCTATATTTG(+CAGTGT)GTACCCGATAGGATACTTTAGGTATCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605192827_rm88 ACTGAATCCTGATACCTAAAGTATCCTATCGG(+CAGTGT)GTACCAAATATAGCTCAGACTTCCACACTCA NGG 10 8 17 8 24 0 100 5 10
    240605192826_wgc4 CCCCAGGAAGGCAGTACCCTGCCCCTGGGAGG(+CAGTGT)GTACCACTCACATGGAACCAGAGGAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605192824_md03 ATGGAGCAGCTTCCTCTGGTTCCATGTGAGTG(+CAGTGT)GTACCCTCCCAGGGGCAGGGTACTGCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605192821_4m54 CCACTCAAGCCAACCGTACAAGCTCCCTGGGG(+CAGTGT)GATCCCACTGGGGAAACCAATGCTATAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605192820_ipc9 CTCTGAAGCTATAGCATTGGTTTCCCCAGTGG(+CAGTGT)GATCCCCCAGGGAGCTTGTACGGTTGGCTTG NGG 10 8 17 8 24 0 100 5 10