Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192741_22g1 AGGGACGGGAGCAAAGAGGCTGCCGTAACTAG(+CAGTGT)GTACCTAGTGGAGGGAAATGGTCACCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605192740_3d4s CATCAGGCTTGGTGACCATTTCCCTCCACTAG(+CAGTGT)GTACCTAGTTACGGCAGCCTCTTTGCTCCCG NGG 10 8 17 8 24 0 100 5 10
    240605192739_gha7 AAAGCTGTCACATGAAAGCCATCCCAGAAATG(+CAGTGT)GTACCTTTTTTTTTTTTTTTTTCCTAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605192738_xvkq ATTCAGTCTTTAGGAAAAAAAAAAAAAAAAAG(+CAGTGT)GTACCATTTCTGGGATGGCTTTCATGTGACA NGG 10 8 17 8 24 0 100 5 10
    240605192736_w6kd GAAGAGCAAACCCATAGTCTTGCCTTCTCCTG(+CAGTGT)GATCCAGAGCTTTCGGAGAACCTAACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605192735_mogt TTTGGTCACAGTTAGGTTCTCCGAAAGCTCTG(+CAGTGT)GATCCAGGAGAAGGCAAGACTATGGGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605192733_hvip GGGCACTGGCGCTGGCCTGCTGCCTGCAGCTG(+CAGTGT)GTACCCTGCCCAAGCTGTCTTCTCTGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605192732_ukk7 CAGGCGGCTGCAGAGAAGACAGCTTGGGCAGG(+CAGTGT)GTACCAGCTGCAGGCAGCAGGCCAGCGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605192730_6xq7 TCTTACCCTCAATGCCAAACAGCTGGCACTGG(+CAGTGT)GTACCTAGGTTCAGGCCAGAAGCCAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605192728_m4ne CTTTCCCTGGCTGGCTTCTGGCCTGAACCTAG(+CAGTGT)GTACCCAGTGCCAGCTGTTTGGCATTGAGGG NGG 10 8 17 8 24 0 100 5 10