Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192632_srl8 ATTGGAAGAGCAACATGGGCCACCCCTGCTGG(+CAGTGT)GTACCCAACTTATGAGCAAGAGAATGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605192631_k5hx TGGAAGCTTCCATTCTCTTGCTCATAAGTTGG(+CAGTGT)GTACCCAGCAGGGGTGGCCCATGTTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605192629_0j2w GCTCTGCGCACAGTTTGTTCACTAGAGGCCTG(+CAGTGT)GTACCTGTCTTCTCTCAGGCCTCTGCACTCA NGG 10 8 17 8 24 0 100 5 10
    240605192628_dnrp CCGTGTGAGTGCAGAGGCCTGAGAGAAGACAG(+CAGTGT)GTACCAGGCCTCTAGTGAACAAACTGTGCGC NGG 10 8 17 8 24 0 100 5 10
    240605192625_bv92 TGGATCAGGAGCCATATTAAGATGACTTTGGG(+CAGTGT)GATCCCAGCAGTCCCTACGTGATCACATAGC NGG 10 8 17 8 24 0 100 5 10
    240605192624_1ux2 CAGGAGCTATGTGATCACGTAGGGACTGCTGG(+CAGTGT)GATCCCCAAAGTCATCTTAATATGGCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605192622_1yn5 ATGATTACTAGCACTAGAAGCCAACAGCAAAG(+CAGTGT)GATCCCGCGCGCAGTTGCTCTTGTTTAATCC NGG 10 8 17 8 24 0 100 5 10
    240605192619_akva AACCTGGATTAAACAAGAGCAACTGCGCGCGG(+CAGTGT)GATCCTTTGCTGTTGGCTTCTAGTGCTAGTA NGG 10 8 17 8 24 0 100 5 10
    240605192616_41ar TGGTCCATTTAAAGTTTCCCAGCTATGGGGTG(+CAGTGT)GTACCAGTTCTGGACAAGTGCCACTGCATCA NGG 10 8 17 8 24 0 100 5 10
    240605192614_qq4d TACTATGATGCAGTGGCACTTGTCCAGAACTG(+CAGTGT)GTACCACCCCATAGCTGGGAAACTTTAAATG NGG 10 8 17 8 24 0 100 5 10