Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192541_5v7e TCACCTTATTTTTGTAGTCTCTCCCTGAACTG(+CAGTGT)GTACCTCACCCATTCAGCTGTCCAGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605192540_rmjx TCTCAAGTTTTCTGGACAGCTGAATGGGTGAG(+CAGTGT)GTACCAGTTCAGGGAGAGACTACAAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605192538_gbz9 AGATGGGGTCTGTGATGGATTGTTACACTGTG(+CAGTGT)GATCCCTGCACTTGGCATCTGCACGAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240605192536_82wj TGGCCTAGACTCGTGCAGATGCCAAGTGCAGG(+CAGTGT)GATCCACAGTGTAACAATCCATCACAGACCC NGG 10 8 17 8 24 0 100 5 10
    240605192535_pm7q AATGTACAGTTGCCTAACAGACAACTTCTAGG(+CAGTGT)GATCCTACAAATTCCCAGGGGATCTGTACTC NGG 10 8 17 8 24 0 100 5 10
    240605192533_g43s ACCTAGAGTACAGATCCCCTGGGAATTTGTAG(+CAGTGT)GATCCCTAGAAGTTGTCTGTTAGGCAACTGT NGG 10 8 17 8 24 0 100 5 10
    240605192530_a3p2 GGATGGACTATCCAGAGACTACCCCACCCAGG(+CAGTGT)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605192529_1d7l AGTGTCTGGGTTTGGTGGCTGATTATGGGATG(+CAGTGT)GATCCCTGGGTGGGGTAGTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240605192527_km2p CATCCTTTATAAGTGGACAATATGTGTATGTG(+CAGTGT)GATCCCGCAAGGCAGGCCAAGTCTTCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605192526_1h8i AATGAGAACTGAAGACTTGGCCTGCCTTGCGG(+CAGTGT)GATCCACATACACATATTGTCCACTTATAAA NGG 10 8 17 8 24 0 100 5 10