Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192524_etpi GGTCCCAGCTACGTGGGCAGCTAAGGCAGGAG(+CAGTGT)GATCCCATTAGCCCATAAGCTCAGGGCTATA NGG 10 8 17 8 24 0 100 5 10
    240605192522_koom CCCAGTATAGCCCTGAGCTTATGGGCTAATGG(+CAGTGT)GATCCTCCTGCCTTAGCTGCCCACGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605192521_dy6c AGCGCCAGCCCTAGGAACCCCAGAAAAGAGTG(+CAGTGT)GTACCTAAGGCTGAAATTGTTTTGCTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605192519_asrm CCCTGGCAACAGCAAAACAATTTCAGCCTTAG(+CAGTGT)GTACCACTCTTTTCTGGGGTTCCTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605192518_o9t2 TCTAAAATTTAAGCCCTTTAAATATCACAAAG(+CAGTGT)GTACCCTTGTTGGCCTTACAAAAGATAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605192516_wt0i AAAGGTAGCTATCTTTTGTAAGGCCAACAAGG(+CAGTGT)GTACCTTTGTGATATTTAAAGGGCTTAAATT NGG 10 8 17 8 24 0 100 5 10
    240605192515_jddp TTTTTATAGCAAAGGGAATGTATCTTAACACG(+CAGTGT)GATCCTGTTTATCTATTGGGACTCCAAACGG NGG 10 8 17 8 24 0 100 5 10
    240605192512_yag0 TTTGACCGTTTGGAGTCCCAATAGATAAACAG(+CAGTGT)GATCCGTGTTAAGATACATTCCCTTTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240605192511_aet3 GTGGTCCCTAGGCAAAAGGAGGGGGATTCTGG(+CAGTGT)GATCCTAAAAGGCTTTATTTTTTGACTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605192509_gcav ACCCCTATGAAAGGGTAGATTGACCTCCAAAG(+CAGTGT)GTACCGCTACCCACAGGCTGAGAACGACTAC NGG 10 8 17 8 24 0 100 5 10