Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192420_loq6 AGGACAAGAATCACCAAAATGCCCATAGTGTG(+CAGTGT)GATCCAAGCCACAGCTTTGACCGCTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605192418_3iu9 GGAGGTGACCCTTAAGGATCTAGATACTGATG(+CAGTGT)GTACCCAGCTGGCAAGTGGCACAGACCACTG NGG 10 8 17 8 24 0 100 5 10
    240605192417_1y0z GAAGCCAGTGGTCTGTGCCACTTGCCAGCTGG(+CAGTGT)GTACCATCAGTATCTAGATCCTTAAGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605192415_2q6d GCTCCCCCAATGCAGGGTAGGGTGCCAGGTGG(+CAGTGT)GATCCAAGACAGCCTTATGTCCTGTAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605192414_zfa0 GGTTCATGTTTACAGGACATAAGGCTGTCTTG(+CAGTGT)GATCCCACCTGGCACCCTACCCTGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240605192412_me2o TATGGATAAAATTGGTGCAAATTGGTCAAACG(+CAGTGT)GATCCAACAAACACTGATGTCCAAGCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605192411_p0he CAATATGCCAGCTTGGACATCAGTGTTTGTTG(+CAGTGT)GATCCGTTTGACCAATTTGCACCAATTTTAT NGG 10 8 17 8 24 0 100 5 10
    240605192410_ug2t ATTCCAAGGCCAAGTCCAGTCTATGAAACAAG(+CAGTGT)GTACCATACCCAGTCTGAGCATGGGATGCTA NGG 10 8 17 8 24 0 100 5 10
    240605192409_1q29 AAAACTAGCATCCCATGCTCAGACTGGGTATG(+CAGTGT)GTACCTTGTTTCATAGACTGGACTTGGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605192407_ezsd GTGCACCACGGGCCGGGCTTCATCTGTGATTG(+CAGTGT)GATCCAGGGAAGAATCAGCGTGAAGTATATT NGG 10 8 17 8 24 0 100 5 10