Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192332_bis6 TAAAGGGCTCATTTCTCTTCTTCTACCTCATG(+CAGTGT)GATCCCGAGGTCTAACTCAGGTCATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605192330_a1n7 GAGGTTCTGGGTTCAATTCCCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605192328_4b97 GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGGAATTGAACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605192326_4urk GCAGGAAGTCCTGAGGTTAGGGACTGGCTATG(+CAGTGT)GTACCCAGGACCCGGTCAGTAGTACCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605192324_xpok TGAGACAGCAGGTACTACTGACCGGGTCCTGG(+CAGTGT)GTACCATAGCCAGTCCCTAACCTCAGGACTT NGG 10 8 17 8 24 0 100 5 10
    240605192323_8ahc GAGAGTGTCTTTTCTGCCTAAGCTTGGCAATG(+CAGTGT)GATCCCGGAAAGAGACGGCTCTCCCGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605192321_jmnh CATGAGCAGCCGGGAGAGCCGTCTCTTTCCGG(+CAGTGT)GATCCATTGCCAAGCTTAGGCAGAAAAGACA NGG 10 8 17 8 24 0 100 5 10
    240605192319_tdwt CTGGCCCTGTGCCTGTGGCACTTTCGCCTATG(+CAGTGT)GTACCATCTCCCCAGTGCAAGTCATGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605192318_lxg1 GGAGGCAGTCCATGACTTGCACTGGGGAGATG(+CAGTGT)GTACCATAGGCGAAAGTGCCACAGGCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605192316_tvy9 TGGAACTCACACCAGGCATAAACAGACAGAGG(+CAGTGT)GATCCGGAACGGCAAGACCTCGGAACACTAC NGG 10 8 17 8 24 0 100 5 10