Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192239_ny2q TTCTCATGGAGACCAACCAGAAGAGGGTGTTG(+CAGTGT)GATCCCCTGGAACTGGAATTACAGATGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605192237_u7a3 AAGATGCCATGCACACTGTCAGAGGACAAAGG(+CAGTGT)GATCCTCAGTGTCACCCAGCTGCAGGTCGCT NGG 10 8 17 8 24 0 100 5 10
    240605192235_z9tj ACACCAGCGACCTGCAGCTGGGTGACACTGAG(+CAGTGT)GATCCCTTTGTCCTCTGACAGTGTGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240605192234_zagc GAACTTGCTGCTAAGCCAGACAGCCTGAGTTG(+CAGTGT)GATCCCTGGGACTCATGGCCTGCTGCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240605192231_9001 AAGACAGCCGGCAGCAGGCCATGAGTCCCAGG(+CAGTGT)GATCCAACTCAGGCTGTCTGGCTTAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605192230_7jn3 TGGACCCCCAAAAGTTCATGCACTGACGGCTG(+CAGTGT)GATCCTCGGGCCGTAGAGTTCAGTGTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605192227_9sto CTCCCATCCTACACTGAACTCTACGGCCCGAG(+CAGTGT)GATCCAGCCGTCAGTGCATGAACTTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605192226_xj8k ATCCTGATTTAGAATAATTGACACTGTTTTAG(+CAGTGT)GTACCCGTTTTCTATTACCTTAAACTATTCA NGG 10 8 17 8 24 0 100 5 10
    240605192224_e46v GATACTGAATAGTTTAAGGTAATAGAAAACGG(+CAGTGT)GTACCTAAAACAGTGTCAATTATTCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605192223_mr76 TAAAGAAAATACAGAACTCCACAGAAAGCTTG(+CAGTGT)GTACCATCTTCCAGCTAACACAGTATAGAGC NGG 10 8 17 8 24 0 100 5 10