Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192222_ca28 CATTGGCTCTATACTGTGTTAGCTGGAAGATG(+CAGTGT)GTACCAAGCTTTCTGTGGAGTTCTGTATTTT NGG 10 8 17 8 24 0 100 5 10
    240605192219_gt6x GACAGCTGGAGGAATGAGCCTTCACTGCCCTG(+CAGTGT)GTACCACTCTAGGCAACTGCATCTGAGACAT NGG 10 8 17 8 24 0 100 5 10
    240605192218_tl87 GGAACATGTCTCAGATGCAGTTGCCTAGAGTG(+CAGTGT)GTACCAGGGCAGTGAAGGCTCATTCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605192217_voih CTCCTTGGCCATAGGAAAGATGCAACTCACAG(+CAGTGT)GTACCAGCTGACATTACAGAGATCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240605192215_he1a TGCAACTGTCCTGATCTCTGTAATGTCAGCTG(+CAGTGT)GTACCTGTGAGTTGCATCTTTCCTATGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605192214_qqbs TGCTCAGTCTATGATGCCCTTGAGCTACCATG(+CAGTGT)GTACCTTGTGCAACATGGCTTGATTAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605192212_6831 CCCAGGAAGCTAATCAAGCCATGTTGCACAAG(+CAGTGT)GTACCATGGTAGCTCAAGGGCATCATAGACT NGG 10 8 17 8 24 0 100 5 10
    240605192210_b4vl TAGTTCTAACTTAAACCACTTGGTGATTGGTG(+CAGTGT)GTACCGTCAGGTGCTGGGCTTCAAAAACTTG NGG 10 8 17 8 24 0 100 5 10
    240605192209_97k0 CTTGCCAAGTTTTTGAAGCCCAGCACCTGACG(+CAGTGT)GTACCACCAATCACCAAGTGGTTTAAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240605192208_j8cq GTGTCAAATTCTGTGAACCAGGGCCAACACTG(+CAGTGT)GTACCAGCTTCACCCTGACCTGGGGCCACTG NGG 10 8 17 8 24 0 100 5 10