Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192206_vssr GAGGGCAGTGGCCCCAGGTCAGGGTGAAGCTG(+CAGTGT)GTACCAGTGTTGGCCCTGGTTCACAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240605192204_yfzg TATAGCTAAGTTTACACCAACCAAATTATTTG(+CAGTGT)GTACCAATTCTTGTAAATACTGGGTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605192203_0nxd TACCCCAGCATACCCAGTATTTACAAGAATTG(+CAGTGT)GTACCAAATAATTTGGTTGGTGTAAACTTAG NGG 10 8 17 8 24 0 100 5 10
    240605192202_flea CTCCCTTGTATCCTGTACAAGACGCCAGCGCG(+CAGTGT)GATCCCACGCTTCGTAAGAAAGGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605192200_5r5k CAAGGAAAGCACTCCTTTCTTACGAAGCGTGG(+CAGTGT)GATCCGCGCTGGCGTCTTGTACAGGATACAA NGG 10 8 17 8 24 0 100 5 10
    240605192159_yzbq TATAGCTAAGTTTACACCAACCAAATTATTTG(+CAGTGT)GTACCAATTCTTGTAAATACTGGGTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605192157_iz6o TACCCCAGCATACCCAGTATTTACAAGAATTG(+CAGTGT)GTACCAAATAATTTGGTTGGTGTAAACTTAG NGG 10 8 17 8 24 0 100 5 10
    240605192156_od5r CTCCCTTGTATCCTGTACAAGACGCCAGCGCG(+CAGTGT)GATCCCACGCTTCGTAAGAAAGGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605192154_onv8 CAAGGAAAGCACTCCTTTCTTACGAAGCGTGG(+CAGTGT)GATCCGCGCTGGCGTCTTGTACAGGATACAA NGG 10 8 17 8 24 0 100 5 10
    240605192153_ov8k TCAGGTTGTCACAGGAATACCCCACTCTGCTG(+CAGTGT)GTACCAATTTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10