Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192137_6oab TTCCAGAAAGGAAAAGCACCAAGAAGCACAGG(+CAGTGT)GTACCCTAACTACTGAGTACCAGAGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605192135_w7q9 TCCCACACTATTAGCTGCTATCAGAAAAACAG(+CAGTGT)GATCCTACAAGTCAAGGTTACTATGTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605192134_8wum TAATTAGGAAACATAGTAACCTTGACTTGTAG(+CAGTGT)GATCCTGTTTTTCTGATAGCAGCTAATAGTG NGG 10 8 17 8 24 0 100 5 10
    240605192131_1k2u ACGAAATGGACAAATTTCTGGACAGATACCAG(+CAGTGT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240605192130_a276 TAGATGGTCAACTTGATCCTGATTCAACTTTG(+CAGTGT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605192128_k23m ACAACAGTCAAAGAAAATACAAAATACAAAAG(+CAGTGT)GATCCTAACTCAAAACATCCAGGAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605192127_2j56 GTGTCCTGGATTTCCTGGATGTTTTGAGTTAG(+CAGTGT)GATCCTTTTGTATTTTGTATTTTCTTTGACT NGG 10 8 17 8 24 0 100 5 10
    240605192125_7s7v TCCTCAGAAAATTGGTCATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACTTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605192124_yxdr TTCTGGATATATGCCCAGGAGAAGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGACCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605192121_2p25 GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10