Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192102_4fax CCTTGGCTTCCTGCTTCCCCCACTTCATCCTG(+CAGTGT)GATCCAGCCCCAGCTTGGTAAGATGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605192101_bbvj TGAGAGGTTCTTTTCTCATCTGTTTTGAATCG(+CAGTGT)GTACCCTTAGTGGAAGGTCTGACGTGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605192059_sbm0 TGCACTTTTCCACGTCAGACCTTCCACTAAGG(+CAGTGT)GTACCGATTCAAAACAGATGAGAAAAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605192058_loqb CCGATAGAGCCCCCGATGCTGAGTGCCAGCAG(+CAGTGT)GATCCTGTGTCCGACACCAGATCTGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240605192056_58v6 ACAAAGATAAACAGATCTGGTGTCGGACACAG(+CAGTGT)GATCCTGCTGGCACTCAGCATCGGGGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605192055_t426 AGGGTCTCATCATATAGACTTACATCTCTGAG(+CAGTGT)GTACCCTCTTGTGGTAATTCATTGTCATTAT NGG 10 8 17 8 24 0 100 5 10
    240605192053_ji7t AGTTGATAATGACAATGAATTACCACAAGAGG(+CAGTGT)GTACCTCAGAGATGTAAGTCTATATGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605192051_0wil TGAGTCACTTTCTATATGTGATAAAGCATGGG(+CAGTGT)GTACCTTCCCACTGGAGCGCAGCAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605192050_0p26 AACACAGAGCCTTGCTGCGCTCCAGTGGGAAG(+CAGTGT)GTACCCCATGCTTTATCACATATAGAAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605192047_z34c CCTGTAAAAACCTGTCCTGTCCTATAGTTGGG(+CAGTGT)GATCCACAATGCCTGGATCTCCTAGGCCCAA NGG 10 8 17 8 24 0 100 5 10