Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192405_huut TGCTGAATATACTTCACGCTGATTCTTCCCTG(+CAGTGT)GATCCAATCACAGATGAAGCCCGGCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240605192403_2l9l AAACATGGGGACTAATAACTTAGAGACAGTTG(+CAGTGT)GTACCGTGTCTAATTTCTGCCTAAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605192402_z5t8 GAAAGCATCTCTTTAGGCAGAAATTAGACACG(+CAGTGT)GTACCAACTGTCTCTAAGTTATTAGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605192400_44ye AAAAAATGGGCTATGAAATTATTTTACATCTG(+CAGTGT)GATCCCTGTATTATAAAGTCTGTCCCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605192359_jnp3 ACGAAGTTTGGGGACAGACTTTATAATACAGG(+CAGTGT)GATCCAGATGTAAAATAATTTCATAGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605192357_kmie TCAGGTCTACATGGAACCTAGAATTTCAGCTG(+CAGTGT)GATCCTGAGAGCTGGTCTTCAACAAGCGAAG NGG 10 8 17 8 24 0 100 5 10
    240605192356_7afd CTGACCTTCGCTTGTTGAAGACCAGCTCTCAG(+CAGTGT)GATCCAGCTGAAATTCTAGGTTCCATGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605192355_c6mo CAGGTGTGGTGGGAATTGTAATCTCAGGACTG(+CAGTGT)GATCCCACCCAACTAAAGTTATTGTCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605192352_my9n GTTAAACACAGACAATAACTTTAGTTGGGTGG(+CAGTGT)GATCCAGTCCTGAGATTACAATTCCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240605192351_4ln3 TAGGGCTTCTCTCCAGTGTGAGTCCTTTCATG(+CAGTGT)GATCCGGAAAGAATGAGAGCAACTGAAGGCC NGG 10 8 17 8 24 0 100 5 10