Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192450_amyr TGCATTTCTCCTTTAACAGCTGGAAAATTAAG(+CAGTGT)GTACCAACCCCGTGCTTCTCTCAGCCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605192448_ak4a TCTTTATCCAGTGCCTGTGATAAACTCTCTGG(+CAGTGT)GATCCAGTGTCCACTTGGGGAAGGTGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605192447_7dch AGCAGAGCCCCACCTTCCCCAAGTGGACACTG(+CAGTGT)GATCCCAGAGAGTTTATCACAGGCACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605192446_qy1y AATTTGGGTGGATGGGTTACTGCATTCTCAAG(+CAGTGT)GATCCATGGCATAGGAAATTGTTCTTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605192444_3qf1 AACTACCCAGAAGAACAATTTCCTATGCCATG(+CAGTGT)GATCCTTGAGAATGCAGTAACCCATCCACCC NGG 10 8 17 8 24 0 100 5 10
    240605192442_3wsn GGATGATTCTACCTCCAGACTTCCTCTTCCTG(+CAGTGT)GATCCATAGCAATATGCAGCTATGGCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605192441_4xip TTTATACAAGGCCATAGCTGCATATTGCTATG(+CAGTGT)GATCCAGGAAGAGGAAGTCTGGAGGTAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605192440_4i63 TTCCCTTCAGTATCTGCTCAGGCAGAAGATGG(+CAGTGT)GTACCCCCACCTCCACCATCATCTTCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605192437_61x1 CGTGGGAGCAGAAGATGATGGTGGAGGTGGGG(+CAGTGT)GTACCCATCTTCTGCCTGAGCAGATACTGAA NGG 10 8 17 8 24 0 100 5 10
    240605192435_mvn5 ATGTTCTGGGATGCCTCCCCTGAAGCCAATGG(+CAGTGT)GTACCAACATCACCAGGAGGAGGAAGCAAAA NGG 10 8 17 8 24 0 100 5 10