Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192611_wg1h GGGCGGGAGTAAGGAAATGCAGGAGTTGACCG(+CAGTGT)GATCCTTGTTCCTCACCTTGTACACTCACTT NGG 10 8 17 8 24 0 100 5 10
    240605192609_4neg AGTTTAAGTGAGTGTACAAGGTGAGGAACAAG(+CAGTGT)GATCCGGTCAACTCCTGCATTTCCTTACTCC NGG 10 8 17 8 24 0 100 5 10
    240605192607_1xae CATGCTATATGTATACTTCTGTTTTCTGTTGG(+CAGTGT)GTACCTTGCAATGCATTCTATGCCTCATTAA NGG 10 8 17 8 24 0 100 5 10
    240605192606_3tat TCCATTTAATGAGGCATAGAATGCATTGCAAG(+CAGTGT)GTACCCAACAGAAAACAGAAGTATACATATA NGG 10 8 17 8 24 0 100 5 10
    240605192604_rpxn CAAGAAAATATCATCCTGAATGAAGTATCCTG(+CAGTGT)GATCCAAAAGGACATGCATGGTGCTTACTCA NGG 10 8 17 8 24 0 100 5 10
    240605192603_enot ATCAGTGAGTAAGCACCATGCATGTCCTTTTG(+CAGTGT)GATCCAGGATACTTCATTCAGGATGATATTT NGG 10 8 17 8 24 0 100 5 10
    240605192601_ts7c GGAATGATTGACCAGTGACTATCCCAATGTGG(+CAGTGT)GATCCAGGAGACTAGCATGGCTGTCCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605192559_cjng GCCTCTCAGAGGACAGCCATGCTAGTCTCCTG(+CAGTGT)GATCCCACATTGGGATAGTCACTGGTCAATC NGG 10 8 17 8 24 0 100 5 10
    240605192558_6q5y GGAAGGAATGAATAGGATGTGGCTAGCAGGAG(+CAGTGT)GATCCTGCAAGCCAAAAGAGCAGCTTAGACA NGG 10 8 17 8 24 0 100 5 10
    240605192557_2cmo AATTTTGTCTAAGCTGCTCTTTTGGCTTGCAG(+CAGTGT)GATCCTCCTGCTAGCCACATCCTATTCATTC NGG 10 8 17 8 24 0 100 5 10