Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192649_x8tg GTTTTGTAGGTCAATTCCTATTTTGTGGGTGG(+CAGTGT)GATCCCCTTTTCATTAGTCTGACATTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605192647_x8ak TCCCAATCTGAATGTCAGACTAATGAAAAGGG(+CAGTGT)GATCCCACCCACAAAATAGGAATTGACCTAC NGG 10 8 17 8 24 0 100 5 10
    240605192646_zqe6 GGATGACACTCCCCGAAAGGGAGAGGGAAATG(+CAGTGT)GTACCAGCGGAAGGCAGATTCTCAGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240605192644_3y73 AAAGAGAAGGTCTGAGAATCTGCCTTCCGCTG(+CAGTGT)GTACCATTTCCCTCTCCCTTTCGGGGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605192643_t0i9 CTTCTTCCTTCTTTTTGTGAACAAATCAATAG(+CAGTGT)GATCCTTTTTACTGTCAGACTTTCCCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605192641_wrrw ACAAATCAAGGGGAAAGTCTGACAGTAAAAAG(+CAGTGT)GATCCTATTGATTTGTTCACAAAAAGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605192640_8br7 TCACAGGTCTTTGTGAGGACTCCAGATCTTGG(+CAGTGT)GATCCACCACTTAGCATTATAATAGATAGCA NGG 10 8 17 8 24 0 100 5 10
    240605192637_51fv GGTCTTGCTATCTATTATAATGCTAAGTGGTG(+CAGTGT)GATCCCAAGATCTGGAGTCCTCACAAAGACC NGG 10 8 17 8 24 0 100 5 10
    240605192636_231g ACTTCAAAAGTTGGTTTTTCTCCTTCTACTGG(+CAGTGT)GATCCAAGGATACAACTCAGGTTATCAGACT NGG 10 8 17 8 24 0 100 5 10
    240605192634_2hsk GAACTAGTCTGATAACCTGAGTTGTATCCTTG(+CAGTGT)GATCCCAGTAGAAGGAGAAAAACCAACTTTT NGG 10 8 17 8 24 0 100 5 10