Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192957_uo5i GCCCCCACAACCTAGCCCAAGAACCAGCCTGG(+CAGTGT)GTACCCATGGATTATATTGGGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605192954_47fe CTCTCTGCTGCCCCTCCCAATATAATCCATGG(+CAGTGT)GTACCCAGGCTGGTTCTTGGGCTAGGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605192951_mc0b CAGGGAATGAGATTGGCCCTTTCTCTGCCAGG(+CAGTGT)GTACCACAGGGACACATGCCGGGTAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605192949_tng8 CATACTCAGGCTACCCGGCATGTGTCCCTGTG(+CAGTGT)GTACCCTGGCAGAGAAAGGGCCAATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605192947_rnxo TTGGTGAGGGTCCTGAGGTGCTCTGGCCACAG(+CAGTGT)GTACCATCCATAGCTCCAACTCATGTGACCA NGG 10 8 17 8 24 0 100 5 10
    240605192945_y5gg CCCTTTGGTCACATGAGTTGGAGCTATGGATG(+CAGTGT)GTACCTGTGGCCAGAGCACCTCAGGACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605192943_it92 ATTAAAAGAGCTTCCTGACAGGGGCCCCTTGG(+CAGTGT)GATCCACCCAAGTGAGACTGCAGAGCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605192941_utoo TCCAAGTTGGGCTCTGCAGTCTCACTTGGGTG(+CAGTGT)GATCCCAAGGGGCCCCTGTCAGGAAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605192938_du9d GCCCCCACAACCTAGCCCAAGAACCAGCCTGG(+CAGTGT)GTACCCATGGATTATATTGGGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605192936_ubw5 CTCTCTGCTGCCCCTCCCAATATAATCCATGG(+CAGTGT)GTACCCAGGCTGGTTCTTGGGCTAGGTTGTG NGG 10 8 17 8 24 0 100 5 10