Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193104_gk80 GGCCCCAGGTGTGCCAGCAGCTCAGGACATGG(+CAGTGT)GATCCAGTGGGCTCTCCTTCCTCTGCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605193101_1tku TGGAGAACCTGCAGAGGAAGGAGAGCCCACTG(+CAGTGT)GATCCCATGTCCTGAGCTGCTGGCACACCTG NGG 10 8 17 8 24 0 100 5 10
    240605193100_8jc5 CTCTATTCCATACTATATCCGTTGTGTGGCTG(+CAGTGT)GTACCTTAGTGGGGAAGGATGTCTCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605193058_n0th GGGCCCATGCTGAGACATCCTTCCCCACTAAG(+CAGTGT)GTACCAGCCACACAACGGATATAGTATGGAA NGG 10 8 17 8 24 0 100 5 10
    240605193057_jxzp TTTTTTTCTAGCATGCTTAGGACCCTGAGTTG(+CAGTGT)GATCCCCAGCACCAGAGGGCAACAGGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605193054_8tsq TCTCTCTCTCCCTGTTGCCCTCTGGTGCTGGG(+CAGTGT)GATCCAACTCAGGGTCCTAAGCATGCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605193052_lm0o AAACAAAAGAGAATGAGAGCAAAGCATGATGG(+CAGTGT)GTACCAAGCAACAAGCAAAGATCAAAATGAA NGG 10 8 17 8 24 0 100 5 10
    240605193051_kutl CACTTTTCATTTTGATCTTTGCTTGTTGCTTG(+CAGTGT)GTACCCATCATGCTTTGCTCTCATTCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605193049_1rzj CCCCCGCCCCCTGTTTTTTCTTTAAGCAACAG(+CAGTGT)GTACCCCGTAGAGGCAGGTAGAATCCTCACA NGG 10 8 17 8 24 0 100 5 10
    240605193048_m15n CAACCTGTGAGGATTCTACCTGCCTCTACGGG(+CAGTGT)GTACCTGTTGCTTAAAGAAAAAACAGGGGGC NGG 10 8 17 8 24 0 100 5 10