Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191916_ehrw ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+CAGTGT)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10
    240605191914_uaz6 CTTTTGTGCACCATTCCCATCTTCTCTGTTAG(+CAGTGT)GATCCCGAGGAGCTCAGAGTTGGACCTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605191912_fche AGACCGGACAGGTCCAACTCTGAGCTCCTCGG(+CAGTGT)GATCCTAACAGAGAAGATGGGAATGGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605191909_3i75 GTCAGCTGATCCGCCGTGCCTGTGGACCCCTG(+CAGTGT)GATCCTTGTACTTTGTACACGTGCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240605191907_ztww CCAGGGTGTGTGGCACGTGTACAAAGTACAAG(+CAGTGT)GATCCAGGGGTCCACAGGCACGGCGGATCAG NGG 10 8 17 8 24 0 100 5 10
    240605191905_pwvj AAGGAGAGTTGGTCTCAGAATCCCCAGGCTGG(+CAGTGT)GATCCTGGCTAGCTGGCCCCTTGTCATCAAG NGG 10 8 17 8 24 0 100 5 10
    240605191903_lb8q TCCTGCTTGATGACAAGGGGCCAGCTAGCCAG(+CAGTGT)GATCCCAGCCTGGGGATTCTGAGACCAACTC NGG 10 8 17 8 24 0 100 5 10
    240605191902_udi3 CACAATGGCAGCTTGTAGCCTTCACCCTTCTG(+CAGTGT)GTACCAGGACTTCATCAACTGAACATTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605191900_0hg5 ATGTATAACAATGTTCAGTTGATGAAGTCCTG(+CAGTGT)GTACCAGAAGGGTGAAGGCTACAAGCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605191857_mrd3 TGCTGAACCTCACTCTCCCTTCAACCTCCCCG(+CAGTGT)GATCCAAACCAGCTCCAGGAGGAGGGACAGG NGG 10 8 17 8 24 0 100 5 10