Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191856_ur6p CACCGCCTGTCCCTCCTCCTGGAGCTGGTTTG(+CAGTGT)GATCCGGGGAGGTTGAAGGGAGAGTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605191854_biav ACAGGTCCGTCCCGGAGCACACAGAGGTGTTG(+CAGTGT)GTACCAAGACTGAAACCTTCACAGATTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605191853_cwuh GCCCAATAGAATCTGTGAAGGTTTCAGTCTTG(+CAGTGT)GTACCAACACCTCTGTGTGCTCCGGGACGGA NGG 10 8 17 8 24 0 100 5 10
    240605191852_g4pk GCAGGTCGGGGTTCTCTCAAGTCCCCCAGTCG(+CAGTGT)GATCCGGGACCCCTTTCTGGGCTAGGAAGCG NGG 10 8 17 8 24 0 100 5 10
    240605191850_b7dz GCGGGCGCTTCCTAGCCCAGAAAGGGGTCCCG(+CAGTGT)GATCCGACTGGGGGACTTGAGAGAACCCCGA NGG 10 8 17 8 24 0 100 5 10
    240605191848_tozo CTGGAGGGATATAAAGGGAACACAGACTTGAG(+CAGTGT)GTACCACAGGCTTTGTCTGAAGAGAGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605191846_qn02 GTGACAGGGACTCTCTTCAGACAAAGCCTGTG(+CAGTGT)GTACCTCAAGTCTGTGTTCCCTTTATATCCC NGG 10 8 17 8 24 0 100 5 10
    240605191844_569e GGTGACCCAAGCCCAGTGACATCCCTTCAGGG(+CAGTGT)GATCCATGTGAGCCTTCCAGGTACTAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605191842_vrt9 CCGCTGTTTCTAGTACCTGGAAGGCTCACATG(+CAGTGT)GATCCCCTGAAGGGATGTCACTGGGCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605191840_krz2 TGACAGCTCACAACTGTCTGAACCCTTCACTG(+CAGTGT)GTACCAGGCACCATGGGGTGCACAGAGAGAT NGG 10 8 17 8 24 0 100 5 10