Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193023_zmwq TTACCACCGAGGTCACTTCACTGAGGTAACTG(+CAGTGT)GTACCTTAGTCTCTTGTGGCCACTTACAGAT NGG 10 8 17 8 24 0 100 5 10
    240605193022_v00f CCGATATCTGTAAGTGGCCACAAGAGACTAAG(+CAGTGT)GTACCAGTTACCTCAGTGAAGTGACCTCGGT NGG 10 8 17 8 24 0 100 5 10
    240605193021_uaoa GCATTCCTCTTGCCCAGCCATGTTCACGGTTG(+CAGTGT)GATCCTTAATGCATTGTGAGTGTTCCATCAA NGG 10 8 17 8 24 0 100 5 10
    240605193019_wplu AAAGTTTGATGGAACACTCACAATGCATTAAG(+CAGTGT)GATCCAACCGTGAACATGGCTGGGCAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605193018_agcl TGAGGTTCATTGCTTAAGTCAATGTGGTAGTG(+CAGTGT)GTACCCACTTATAATTCCAGTGTTTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605193016_ylg6 CTCAGCCTCCCAAACACTGGAATTATAAGTGG(+CAGTGT)GTACCACTACCACATTGACTTAAGCAATGAA NGG 10 8 17 8 24 0 100 5 10
    240605193015_n8ok TCTCCTCCCCCTCGACCCTCCTAAATAACACG(+CAGTGT)GTACCAGGCTTGCTGGCTGTGGGATTTACAG NGG 10 8 17 8 24 0 100 5 10
    240605193013_fb0n TATACCTGTAAATCCCACAGCCAGCAAGCCTG(+CAGTGT)GTACCGTGTTATTTAGGAGGGTCGAGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605193010_vthp TTGGTGGGAAGTCTGCTGGAAGAGGCTTCCGG(+CAGTGT)GTACCATTTAGGGGAAGTCAAACTTGTGACT NGG 10 8 17 8 24 0 100 5 10
    240605193008_qpu3 TTTATAGTCACAAGTTTGACTTCCCCTAAATG(+CAGTGT)GTACCCGGAAGCCTCTTCCAGCAGACTTCCC NGG 10 8 17 8 24 0 100 5 10