Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191745_x4t2 GCTTGTGTCAGGTTGACGCACAAAACCAGCCG(+CAGTGT)GTACCGTGTGCATCAGAACTATGAAAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605191744_tkxi CTCAGGAATGGGATTGGTGTGATGCCATAATG(+CAGTGT)GTACCTGCCCTGTCCAAGGACAGCCTGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605191743_2f3l TGTATGACCCAGGCTGTCCTTGGACAGGGCAG(+CAGTGT)GTACCATTATGGCATCACACCAATCCCATTC NGG 10 8 17 8 24 0 100 5 10
    240605191741_akjm GGACACAGTAACCTTGGGTGGTGTGTTCTAGG(+CAGTGT)GTACCTGCCTTCCCGGAAGGCTCAGGGACTA NGG 10 8 17 8 24 0 100 5 10
    240605191739_2obb CTTGCTAGTCCCTGAGCCTTCCGGGAAGGCAG(+CAGTGT)GTACCCTAGAACACACCACCCAAGGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240605191738_vjiq TTCAAAGTAACACATTTACCAGGCAAGGCAAG(+CAGTGT)GTACCAGCAGTGATGGAGGAGACTGTATGGA NGG 10 8 17 8 24 0 100 5 10
    240605191736_y0nr AGTCATCCATACAGTCTCCTCCATCACTGCTG(+CAGTGT)GTACCTTGCCTTGCCTGGTAAATGTGTTACT NGG 10 8 17 8 24 0 100 5 10
    240605191735_zs6k TCCCCCTCCCTTCTACCTTTTGCTTGAGACCG(+CAGTGT)GATCCCTCTCTCTATAGCCCAGCCTGGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605191733_zruy AGTTCAAGGCCAGGCTGGGCTATAGAGAGAGG(+CAGTGT)GATCCGGTCTCAAGCAAAAGGTAGAAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605191732_0dp5 GTGGAATGTGGCCCTAGGATGTACTGGTTTAG(+CAGTGT)GATCCTAGGAAGGTAATCAATCATCCTCAGA NGG 10 8 17 8 24 0 100 5 10