Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191650_8uin CACAGCCACTTTACGGAAAGACAACCAGGCTG(+CAGTGT)GATCCCAGCATCTCGCGGGAAGCGACTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605191649_o9og TTTTCCCATCCCAGCTTCCTTCTGGTAAACTG(+CAGTGT)GTACCCTCAGGATAAAGAAGTATATGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605191647_0i4b AGGCAGGCACCATATACTTCTTTATCCTGAGG(+CAGTGT)GTACCAGTTTACCAGAAGGAAGCTGGGATGG NGG 10 8 17 8 24 0 100 5 10
    240605191645_qr5i AATAATTTTTTTACAAAAAAAATTCTATCAAG(+CAGTGT)GATCCTTTAAATATCAAGTTTCCCAATGCAC NGG 10 8 17 8 24 0 100 5 10
    240605191644_xsqf TCTAAGTGCATTGGGAAACTTGATATTTAAAG(+CAGTGT)GATCCTTGATAGAATTTTTTTTGTAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605191642_9nby AAAAGAGGCAAACCCACAAGTCCCTGCCTTGG(+CAGTGT)GATCCTAGTTCCTAGCAGGGGTCAGCTTATC NGG 10 8 17 8 24 0 100 5 10
    240605191639_w8t2 CCTTGGATAAGCTGACCCCTGCTAGGAACTAG(+CAGTGT)GATCCCAAGGCAGGGACTTGTGGGTTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605191636_nof3 CACTGTAGATGCTACAAACATGTCTACCCAGG(+CAGTGT)GTACCACTGAGCTACGTCTTCCTCATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605191635_kw3n CGTCTGCCCCATGAGGAAGACGTAGCTCAGTG(+CAGTGT)GTACCCTGGGTAGACATGTTTGTAGCATCTA NGG 10 8 17 8 24 0 100 5 10
    240605191633_nyzj TTTGCAGCTGTCAGTTGTTCTTTAAACTCTAG(+CAGTGT)GATCCATCTTTGGACCAAAAGTCTGGTCTGG NGG 10 8 17 8 24 0 100 5 10