Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191730_0349 GACACTCTGAGGATGATTGATTACCTTCCTAG(+CAGTGT)GATCCTAAACCAGTACATCCTAGGGCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605191728_yr65 TGGACATTCGGGCACAGATAAGATGCTTGCAG(+CAGTGT)GATCCTGTTAGGGTTTTTCTTGCTGGTTCCG NGG 10 8 17 8 24 0 100 5 10
    240605191727_26iu ATCAACGGAACCAGCAAGAAAAACCCTAACAG(+CAGTGT)GATCCTGCAAGCATCTTATCTGTGCCCGAAT NGG 10 8 17 8 24 0 100 5 10
    240605191724_7fyn TCTCTCTCTTCAGACACCAGAAGGTGGCATAG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605191722_v9q9 ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCTATGCCACCTTCTGGTGTCTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605191721_u9j1 GGGTGGGGGCGGGATAGGGGCAGTTAAACAAG(+CAGTGT)GTACCCTGGGGAAGAAATGAAGGTCAGATCC NGG 10 8 17 8 24 0 100 5 10
    240605191718_orwn CCATTGGATCTGACCTTCATTTCTTCCCCAGG(+CAGTGT)GTACCTTGTTTAACTGCCCCTATCCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605191716_o8o7 TTTCAGGACACCTTGTGTCATGTGTGTTCAGG(+CAGTGT)GATCCTAACTTGGATCTTCATGATTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605191715_wogf TCTGACTTTGCAATCATGAAGATCCAAGTTAG(+CAGTGT)GATCCCTGAACACACATGACACAAGGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605191714_p1md AACCTTCCATAGTCTGAGCTTTACAGGAGCAG(+CAGTGT)GATCCCCAGCCATCTTCCCTCACTCCTCCAG NGG 10 8 17 8 24 0 100 5 10