Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191822_np2z TGTGCCAACCGGTACACCTGACTCAGCATTAG(+CAGTGT)GATCCTATAACCATGCCTGTCCCTGCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605191820_ycf8 TCAGTAGATACCACAATGAAGCTGAGCTGGGG(+CAGTGT)GATCCTGGGGCCACATTAGTCAGACAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605191817_9a6l AGGGACCAGCTGTCTGACTAATGTGGCCCCAG(+CAGTGT)GATCCCCCAGCTCAGCTTCATTGTGGTATCT NGG 10 8 17 8 24 0 100 5 10
    240605191814_o3jf ATGGGCTCATCTGTGCATAGACTCAGCCCTGG(+CAGTGT)GATCCACAGAGAGGCAGATCGCTATGCCTTA NGG 10 8 17 8 24 0 100 5 10
    240605191812_90gj CTCTGTAAGGCATAGCGATCTGCCTCTCTGTG(+CAGTGT)GATCCCAGGGCTGAGTCTATGCACAGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605191811_l7y5 TGCAGGTTTTGAAAGATGTGTCTCATTATCTG(+CAGTGT)GTACCATTCCTGGATCCTCACATTACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605191810_cogf CTTTTAAGAAGTAATGTGAGGATCCAGGAATG(+CAGTGT)GTACCAGATAATGAGACACATCTTTCAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605191807_zgef ACAGGGAAGTTCAAAAGGCACACAGTTGGGGG(+CAGTGT)GATCCTCTATCAGGGGTAGTATAAGTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605191806_7ccl AACTTTCTTAACTTATACTACCCCTGATAGAG(+CAGTGT)GATCCCCCCAACTGTGTGCCTTTTGAACTTC NGG 10 8 17 8 24 0 100 5 10
    240605191803_epux CTAGGGTCCCATCGTTTGGTTAAATACCACGG(+CAGTGT)GTACCAGTTAACTCCTGGGCTCTAACCTAGA NGG 10 8 17 8 24 0 100 5 10