Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190230_blku GGTGTCAGCGTTTGCATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605190228_tjng GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATGCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605190227_rn63 ATGTTTATAATTTTATATGAGCTACATTCTAG(+CAGTGT)GTACCCTTAAACAAGTTTGATACATGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605190225_900z ACCCATGACCCATGTATCAAACTTGTTTAAGG(+CAGTGT)GTACCTAGAATGTAGCTCATATAAAATTATA NGG 10 8 17 8 24 0 100 5 10
    240605190223_qzxe ATTTTGCCATGTGGCTGGTTACCTGTGTTTGG(+CAGTGT)GTACCACACATCAGTCTCCTCATGTCTTACC NGG 10 8 17 8 24 0 100 5 10
    240605190222_2dth CGCCTGGTAAGACATGAGGAGACTGATGTGTG(+CAGTGT)GTACCCAAACACAGGTAACCAGCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240605190220_g76n GTAACGCCCCAGCCGCAAGGCTGCCTCGCTGG(+CAGTGT)GTACCCATGTGGCAACCAGCGATGTGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605190219_w2b7 GCAACCTGACCACATCGCTGGTTGCCACATGG(+CAGTGT)GTACCCAGCGAGGCAGCCTTGCGGCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605190218_qmvs CTGGCTTCTGAAACAAGCAAACCCCTTTAAAG(+CAGTGT)GTACCCTGGGAAGTCAAGTTTGCTAGCAGTC NGG 10 8 17 8 24 0 100 5 10
    240605190214_63b8 TCTCAGACTGCTAGCAAACTTGACTTCCCAGG(+CAGTGT)GTACCTTTAAAGGGGTTTGCTTGTTTCAGAA NGG 10 8 17 8 24 0 100 5 10