Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190125_0c4w GTAAATGCTAACAGGGGTGGGAGGCTCTGGTG(+CAGTGT)GATCCTGACAGACACAACCCATAGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240605190123_x81w TGGTTGCAGTGTCTATGGGTTGTGTCTGTCAG(+CAGTGT)GATCCACCAGAGCCTCCCACCCCTGTTAGCA NGG 10 8 17 8 24 0 100 5 10
    240605190122_2xty TCTCTGTTAAGTCGATGTGGACTCCAGTCCAG(+CAGTGT)GTACCTGAATGGTTTACCTACGCACAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605190121_lxww CACCGGGCTCTGTGCGTAGGTAAACCATTCAG(+CAGTGT)GTACCTGGACTGGAGTCCACATCGACTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605190120_t536 TTAGGATCTAGACCTCTCCTCTGAGCGCTCTG(+CAGTGT)GATCCAAATCTTAGCACCAGCTTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605190118_3f91 GCGAAGAAGGAAGAAGCTGGTGCTAAGATTTG(+CAGTGT)GATCCAGAGCGCTCAGAGGAGAGGTCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605190117_s2o4 CTCCCAAAGATGGCAAGGTCTAACCATACATG(+CAGTGT)GATCCTCTGGCTTTGGTTTCTGCGTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605190115_75li AGTATTACTTAACGCAGAAACCAAAGCCAGAG(+CAGTGT)GATCCATGTATGGTTAGACCTTGCCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240605190114_ma71 ATTTGTTTTATATGAAATAATTTATTTTTCAG(+CAGTGT)GTACCATTTTTTTTTTCATTTTAACTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605190113_7sxi GTAAAAACAAAGTTAAAATGAAAAAAAAAATG(+CAGTGT)GTACCTGAAAAATAAATTATTTCATATAAAA NGG 10 8 17 8 24 0 100 5 10