Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190110_u9xf CCTACTGGGACCCGAGGTAACTCCTGGGGAGG(+CAGTGT)GATCCCTGGGGTTCATCATACTCAAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605190108_8u5b TTCCTGACCCCTTGAGTATGATGAACCCCAGG(+CAGTGT)GATCCCTCCCCAGGAGTTACCTCGGGTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605190105_3kym GTCCACTGAGGTGGGGAGAGTGTGCTGGGGGG(+CAGTGT)GATCCAGATGCTAGAGCCACACACAAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605190104_c8ae CATTACCCCTTTGTGTGTGGCTCTAGCATCTG(+CAGTGT)GATCCCCCCCAGCACACTCTCCCCACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605190102_fjrt TCGGTCGGACATGGAGTAGTTTGCTTTCCTGG(+CAGTGT)GTACCCCTTGCTACAGCACCGTTGTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605190059_pexb GTGCACACAGGACAACGGTGCTGTAGCAAGGG(+CAGTGT)GTACCCAGGAAAGCAAACTACTCCATGTCCG NGG 10 8 17 8 24 0 100 5 10
    240605190057_4m40 CATGAGGAGCACACAAGAGGGCCTCCTCAGGG(+CAGTGT)GTACCCTGCTCCAGCCCCCAGGCTTTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605190056_8zkf AAGTGTTAAAAAAGCCTGGGGGCTGGAGCAGG(+CAGTGT)GTACCCCTGAGGAGGCCCTCTTGTGTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605190054_olbi GGCAGGGGTGGGAGTGAGGCACAGAAGCGTGG(+CAGTGT)GATCCCCAGCTAGGAATGTGGGTGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605190052_jnoo CTTATGAGCAAGCACCCACATTCCTAGCTGGG(+CAGTGT)GATCCCACGCTTCTGTGCCTCACTCCCACCC NGG 10 8 17 8 24 0 100 5 10