Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190017_maw3 CACCACCGGGATCTGTCCTGCAAAGTTGAAGG(+CAGTGT)GATCCTTTGGTGCCAGCTGGTGTTTGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605190015_3upk TTCCTACTTCCAAACACCAGCTGGCACCAAAG(+CAGTGT)GATCCCTTCAACTTTGCAGGACAGATCCCGG NGG 10 8 17 8 24 0 100 5 10
    240605190012_ahv9 CCTCTCATGGCGCTGCTACCGATGACTCCCAG(+CAGTGT)GATCCCAGACGTTCAGAACCAGATTCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605190010_5kmq AAAGCAATGAGAATCTGGTTCTGAACGTCTGG(+CAGTGT)GATCCTGGGAGTCATCGGTAGCAGCGCCATG NGG 10 8 17 8 24 0 100 5 10
    240605190008_afxs CCTGGCAGGCAGGTGGGCGGTCTGCCTCCAGG(+CAGTGT)GATCCTGGGACCCTGATGGCGATCGTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605190004_4d0o GCATGACAGGACGATCGCCATCAGGGTCCCAG(+CAGTGT)GATCCCTGGAGGCAGACCGCCCACCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605190002_2blv TACTCATCTTGTACCAAATTGTCTGATGGCAG(+CAGTGT)GATCCAGAAGGTATTTTGTCAGCCCAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605190001_7px0 TATATGGAGCTGGGCTGACAAAATACCTTCTG(+CAGTGT)GATCCTGCCATCAGACAATTTGGTACAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605190000_km31 TCTGAAGGATGCCCTGGGATAGATGCAGAATG(+CAGTGT)GTACCTAGGAACCTGCTGACATTTTGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605185958_hvoo CACCCTTCTGCAAAATGTCAGCAGGTTCCTAG(+CAGTGT)GTACCATTCTGCATCTATCCCAGGGCATCCT NGG 10 8 17 8 24 0 100 5 10