Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185906_wiu0 CTTTGCCCATGTATCTGCTCCTACAGCGGGTG(+CAGTGT)GTACCCACATCACTCAAGTACATATCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240605185904_yjm6 GTGACAGCCGGATATGTACTTGAGTGATGTGG(+CAGTGT)GTACCACCCGCTGTAGGAGCAGATACATGGG NGG 10 8 17 8 24 0 100 5 10
    240605185902_ag0g ACACTCAATGCACTTTATTACACAAAATGAGG(+CAGTGT)GATCCGTAAACAGGGCAGGACCCAAGAGTCC NGG 10 8 17 8 24 0 100 5 10
    240605185901_1ins GCTCAGGACTCTTGGGTCCTGCCCTGTTTACG(+CAGTGT)GATCCCTCATTTTGTGTAATAAAGTGCATTG NGG 10 8 17 8 24 0 100 5 10
    240605185859_ordo GTAGGTCGAGCTAGAGGGCTACAGAGAGGCAG(+CAGTGT)GATCCAGGATTAGAGAAATTCCTAAGATCTG NGG 10 8 17 8 24 0 100 5 10
    240605185858_px9a TCTGGCAGATCTTAGGAATTTCTCTAATCCTG(+CAGTGT)GATCCTGCCTCTCTGTAGCCCTCTAGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605185856_rdqb GCAGTACTCTGAAGCAGGCAAATGGGTCTTAG(+CAGTGT)GATCCCTCCCAGAAACCTGCTCTGACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605185854_88v2 ATGGGTCTGGGTCAGAGCAGGTTTCTGGGAGG(+CAGTGT)GATCCTAAGACCCATTTGCCTGCTTCAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605185853_dnyq TCCCATGGGAATTTTCGTCTGTTAATACTGTG(+CAGTGT)GATCCGGTCTGTTTATATGGGGAATGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605185851_5q7l GAAGTCAGCACATTCCCCATATAAACAGACCG(+CAGTGT)GATCCACAGTATTAACAGACGAAAATTCCCA NGG 10 8 17 8 24 0 100 5 10