Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185817_dz2o ACCGCAGTTCCAGAAGCTTCGCCCTTCACAGG(+CAGTGT)GATCCACGCCCTCACTCGCTGTGTCACATGA NGG 10 8 17 8 24 0 100 5 10
    240605185815_tzpc GCTCCTCATGTGACACAGCGAGTGAGGGCGTG(+CAGTGT)GATCCCTGTGAAGGGCGAAGCTTCTGGAACT NGG 10 8 17 8 24 0 100 5 10
    240605185813_0x1n CATGTTTGGAAAGCCCCACCCCTACAGCCCTG(+CAGTGT)GATCCTCTGTTCAGAATCAAAGCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605185812_vaar GCAGGGAAGGGCTGCTTTGATTCTGAACAGAG(+CAGTGT)GATCCAGGGCTGTAGGGGTGGGGCTTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605185810_3t9p AACACAAGGCTGCCATCCTGCAGCTCCAGAGG(+CAGTGT)GATCCCAGCCCTGGACCAAGGTCTGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605185808_jo5a TACTTAGTTGCCAGACCTTGGTCCAGGGCTGG(+CAGTGT)GATCCCTCTGGAGCTGCAGGATGGCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605185807_qx5o TTTTCAATTATAAGCAAGACAGGTGCAGACAG(+CAGTGT)GTACCATGAGACCCTCAGAGGCAGGAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605185806_fflh CCGCCAGCACTCCTGCCTCTGAGGGTCTCATG(+CAGTGT)GTACCTGTCTGCACCTGTCTTGCTTATAATT NGG 10 8 17 8 24 0 100 5 10
    240605185805_aroj GGTTCCCTTCCAGTATGCAGTTAATCATGATG(+CAGTGT)GATCCTGTATATACGAGCTCCTCCGTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240605185804_625k GCTGAAGGGGACGGAGGAGCTCGTATATACAG(+CAGTGT)GATCCATCATGATTAACTGCATACTGGAAGG NGG 10 8 17 8 24 0 100 5 10