Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185802_h7nm GGGAGGCTGTAAAATGGCCCATGTATATGTAG(+CAGTGT)GATCCTGGATGGTCTACATATATGTTAATAC NGG 10 8 17 8 24 0 100 5 10
    240605185800_gaog TTCATGTATTAACATATATGTAGACCATCCAG(+CAGTGT)GATCCTACATATACATGGGCCATTTTACAGC NGG 10 8 17 8 24 0 100 5 10
    240605185758_1glq GGACACCCTGTAACCTTACTGCTTCAGGGCTG(+CAGTGT)GATCCAGACTAAGAGGTTGTGAGGTTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605185757_vce5 CTGCATCCCCAACCTCACAACCTCTTAGTCTG(+CAGTGT)GATCCAGCCCTGAAGCAGTAAGGTTACAGGG NGG 10 8 17 8 24 0 100 5 10
    240605185756_k05v TTATAGGGAAGATGATGTTAACACCTACTTTG(+CAGTGT)GTACCTTGACAAGTGTTACAGAGATCAACAA NGG 10 8 17 8 24 0 100 5 10
    240605185754_6kos CCCTTTTGTTGATCTCTGTAACACTTGTCAAG(+CAGTGT)GTACCAAAGTAGGTGTTAACATCATCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605185753_0fgy GCTCCTTCCCTGTATCTCTCAGGCAGCAAGTG(+CAGTGT)GATCCCTCCGGACTAAAGGCCTAAGGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605185751_u848 GTCCCTGAAACCTTAGGCCTTTAGTCCGGAGG(+CAGTGT)GATCCACTTGCTGCCTGAGAGATACAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605185750_jfet TTTATAGCAAGAACAGTTCCCACTCCGAGCAG(+CAGTGT)GATCCTAAACTCCATCCCAAGTGAATACAGA NGG 10 8 17 8 24 0 100 5 10
    240605185748_y3th AGAGATCTGTATTCACTTGGGATGGAGTTTAG(+CAGTGT)GATCCTGCTCGGAGTGGGAACTGTTCTTGCT NGG 10 8 17 8 24 0 100 5 10