Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185753_0fgy GCTCCTTCCCTGTATCTCTCAGGCAGCAAGTG(+CAGTGT)GATCCCTCCGGACTAAAGGCCTAAGGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605185751_u848 GTCCCTGAAACCTTAGGCCTTTAGTCCGGAGG(+CAGTGT)GATCCACTTGCTGCCTGAGAGATACAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605185750_jfet TTTATAGCAAGAACAGTTCCCACTCCGAGCAG(+CAGTGT)GATCCTAAACTCCATCCCAAGTGAATACAGA NGG 10 8 17 8 24 0 100 5 10
    240605185748_y3th AGAGATCTGTATTCACTTGGGATGGAGTTTAG(+CAGTGT)GATCCTGCTCGGAGTGGGAACTGTTCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605185746_1w7h CCAGGCCGTGCTGGGGTGCTCTCTCTGCTGGG(+CAGTGT)GATCCTTATGGGGCCGGAGGCCACCTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605185745_qwbr ATGCTTTCCTAGGTGGCCTCCGGCCCCATAAG(+CAGTGT)GATCCCCAGCAGAGAGAGCACCCCAGCACGG NGG 10 8 17 8 24 0 100 5 10
    240605185744_ekju TCTATGAGTATCTGTAAATGTTTTCTGAAGAG(+CAGTGT)GATCCACTGAGCAGCTGTAACCCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605185743_al9k CCACACTGGAGATGGGTTACAGCTGCTCAGTG(+CAGTGT)GATCCTCTTCAGAAAACATTTACAGATACTC NGG 10 8 17 8 24 0 100 5 10
    240605185741_pr78 GTTCAAGGTCAGAGAGGTCTGGGTGTGGGCTG(+CAGTGT)GATCCCATGCAGATGTTTGGTAGGTTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605185739_otve TGGGGTGCTCAACCTACCAAACATCTGCATGG(+CAGTGT)GATCCAGCCCACACCCAGACCTCTCTGACCT NGG 10 8 17 8 24 0 100 5 10