Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185641_np65 AACGTTCTGTACCAAACTGCCTGGAGGTTCTG(+CAGTGT)GATCCTCGCAGCTCTCGTAGTCATTGGGCGC NGG 10 8 17 8 24 0 100 5 10
    240605185640_ldy6 CGGCTGCGCCCAATGACTACGAGAGCTGCGAG(+CAGTGT)GATCCAGAACCTCCAGGCAGTTTGGTACAGA NGG 10 8 17 8 24 0 100 5 10
    240605185639_rbat ACAGACCCAGAGGGGGCGAGCCCTTGCTGCAG(+CAGTGT)GTACCAATTGGCTGAGCCATAGATCCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605185638_z0zt TGGTGCCCCTGGATCTATGGCTCAGCCAATTG(+CAGTGT)GTACCTGCAGCAAGGGCTCGCCCCCTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605185636_6sll AAGCTTTCTTAGAACTTGTTTTTATTCTTTGG(+CAGTGT)GTACCCAAAGGCCAAGTGATGAATCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605185634_bsew AATTCTTGCCAGATTCATCACTTGGCCTTTGG(+CAGTGT)GTACCCAAAGAATAAAAACAAGTTCTAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605185632_t4xy TGTGGGAGAAGAGCCCTGACCAAGCTGATCCG(+CAGTGT)GTACCTTGAAGGAAGTGCAATTGTTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240605185630_q75q AGTATGCATGAAACAATTGCACTTCCTTCAAG(+CAGTGT)GTACCGGATCAGCTTGGTCAGGGCTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605185629_gagi AGCTAGAGTCTGGGTTGGAGAGGCCGAGTGAG(+CAGTGT)GATCCTTACAAGGCTACTGCATAGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605185627_ep1o TCCCGTGAGAAGCTATGCAGTAGCCTTGTAAG(+CAGTGT)GATCCTCACTCGGCCTCTCCAACCCAGACTC NGG 10 8 17 8 24 0 100 5 10