Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185552_sz6u CACGCGCCGCTCTGCTCCGGAGCTCTGCGAAG(+CAGTGT)GATCCCGACCGCGCCGGGCTATGCAGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605185551_bhse CTGCAGCTGCCTGCATAGCCCGGCGCGGTCGG(+CAGTGT)GATCCTTCGCAGAGCTCCGGAGCAGAGCGGC NGG 10 8 17 8 24 0 100 5 10
    240605185549_afd0 AGTCTCCAGATTCAGGTAACAGCTTGATATGG(+CAGTGT)GATCCATAAGTGTCAAATTGGGCTTTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605185548_jlo8 TTTTTCACACAAAGCCCAATTTGACACTTATG(+CAGTGT)GATCCCATATCAAGCTGTTACCTGAATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605185546_lue2 TCTCTTATGAAGTCAGGCCGGCCAGGGAAGGG(+CAGTGT)GATCCCATAGATTCCTATGAAAGCTATCAAG NGG 10 8 17 8 24 0 100 5 10
    240605185544_3d5f AGCATCTTGATAGCTTTCATAGGAATCTATGG(+CAGTGT)GATCCCCTTCCCTGGCCGGCCTGACTTCATA NGG 10 8 17 8 24 0 100 5 10
    240605185542_v49h GGAATTTGTGACAGCAGCCCTAGAAAACCAGG(+CAGTGT)GTACCTTCCACCTCCCCATCCCTCCATCTCA NGG 10 8 17 8 24 0 100 5 10
    240605185540_lvg2 AAGGATGAGATGGAGGGATGGGGAGGTGGAAG(+CAGTGT)GTACCCTGGTTTTCTAGGGCTGCTGTCACAA NGG 10 8 17 8 24 0 100 5 10
    240605185539_8khu AGCGGCTCAGTGCCTGATGCCAGCAGCGGAAG(+CAGTGT)GTACCGTTTCCCACACGACAGACAGCCACAC NGG 10 8 17 8 24 0 100 5 10
    240605185537_ly8c CAACTGTGTGGCTGTCTGTCGTGTGGGAAACG(+CAGTGT)GTACCTTCCGCTGCTGGCATCAGGCACTGAG NGG 10 8 17 8 24 0 100 5 10