Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185610_vdk4 GCCACTGAACCGACAGAGTTGCCCTAAGCCGG(+CAGTGT)GTACCTGACCTGAGTTCAGGGTAGCAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605185608_wko5 CTGTCTAGGCTGCTACCCTGAACTCAGGTCAG(+CAGTGT)GTACCCGGCTTAGGGCAACTCTGTCGGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605185606_dlir TTAGTGTTCACAGCAGTTAGAACTCACCCCTG(+CAGTGT)GATCCTGCAACCCATTTCCACTGATGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605185604_me08 ATTTTAACCACATCAGTGGAAATGGGTTGCAG(+CAGTGT)GATCCAGGGGTGAGTTCTAACTGCTGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605185602_mykn GTCACCCCAAACCAAAGCGAAACACAGACCTG(+CAGTGT)GTACCGAAGACACTTCGTATTCCCAAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240605185601_5pt2 GCACACGGCTTTGGGAATACGAAGTGTCTTCG(+CAGTGT)GTACCAGGTCTGTGTTTCGCTTTGGTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605185559_zhd3 GTTTGTCTGGGTGTAGGGTTGGACAGCTCTGG(+CAGTGT)GATCCGATTAAGGCCATGTTCTGGGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605185557_kc2n TGAGGGGCAGCCCCAGAACATGGCCTTAATCG(+CAGTGT)GATCCCAGAGCTGTCCAACCCTACACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605185555_kwii ATGCTGCCTGTGAAGCTGTGTTCTCAGGTCAG(+CAGTGT)GATCCTGTTCACCAGCGTCAGAAAGTGTACA NGG 10 8 17 8 24 0 100 5 10
    240605185554_d48m TGGTGTGTACACTTTCTGACGCTGGTGAACAG(+CAGTGT)GATCCTGACCTGAGAACACAGCTTCACAGGC NGG 10 8 17 8 24 0 100 5 10