Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185718_ufdl GAACTCTCCCTGGGTTCAGCCCTCTGGGGCGG(+CAGTGT)GTACCTCAGAGTGGGGGCTGGGTCACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605185717_cg67 TCTCTCTTGGGTGACCCAGCCCCCACTCTGAG(+CAGTGT)GTACCCGCCCCAGAGGGCTGAACCCAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605185715_jcah GGAGGGAGCTCCCCTGCACCGAGTAACTGGTG(+CAGTGT)GATCCCTGAAGTCACGGCAGCTCCGCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605185713_wsyv CCTCCGCCTTGCGGAGCTGCCGTGACTTCAGG(+CAGTGT)GATCCACCAGTTACTCGGTGCAGGGGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605185711_jq6f CCCAGGTCTGGAACTTAGTAGACTGGGGTTGG(+CAGTGT)GTACCCAACCAATCAAACCCCTCCAAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605185710_oq1o GTTCCAGGCTTTGGAGGGGTTTGATTGGTTGG(+CAGTGT)GTACCCAACCCCAGTCTACTAAGTTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605185707_umq8 TGTGAACGAGACAATGGAGCAAGGGGGAGGGG(+CAGTGT)GTACCTTCCATTTCCCAGCAGTTGTTCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605185705_9xnf ACCAGACTGGAACAACTGCTGGGAAATGGAAG(+CAGTGT)GTACCCCCTCCCCCTTGCTCCATTGTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240605185703_y317 ATCAGAAGACACCCTCAGGTGTCACTCCTTAG(+CAGTGT)GTACCACCCACTTACAGTTTTGAGATGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605185702_of2j GAGAGAACTCATCTCAAAACTGTAAGTGGGTG(+CAGTGT)GTACCTAAGGAGTGACACCTGAGGGTGTCTT NGG 10 8 17 8 24 0 100 5 10