Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185738_tt4a GGGGAGGTGAGACAGCTCAAATTTTGATCATG(+CAGTGT)GATCCAACCTCACGGGCACTAGACAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605185735_mvol GTGAAACACTCTGTCTAGTGCCCGTGAGGTTG(+CAGTGT)GATCCATGATCAAAATTTGAGCTGTCTCACC NGG 10 8 17 8 24 0 100 5 10
    240605185732_jcax CAATCCTATCATGCAAGATTGAGGCCCTTAGG(+CAGTGT)GTACCTGACAACGGGCACTAATACACCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605185731_x9ha CAGCCATGGGGTGTATTAGTGCCCGTTGTCAG(+CAGTGT)GTACCCTAAGGGCCTCAATCTTGCATGATAG NGG 10 8 17 8 24 0 100 5 10
    240605185729_63cn CCCTGAATAGTCTGTCTGGCTTTCCTTGCCAG(+CAGTGT)GATCCATCAGTGGTGTTTGTTTTTTTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605185728_y7re AGCACCAAGAAAAAAAACAAACACCACTGATG(+CAGTGT)GATCCTGGCAAGGAAAGCCAGACAGACTATT NGG 10 8 17 8 24 0 100 5 10
    240605185726_ukjt CAGTGAGCCAAAGCCCAGCGCCAGTTGTTGAG(+CAGTGT)GATCCAGAAATCGAGATGTCTGATACCACTA NGG 10 8 17 8 24 0 100 5 10
    240605185725_ox88 TGCCTTAGTGGTATCAGACATCTCGATTTCTG(+CAGTGT)GATCCTCAACAACTGGCGCTGGGCTTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605185723_xwqd CAAGAGTGGTGGTTGGGCAAGGAGGGCCCTTG(+CAGTGT)GTACCTAGACCATGGTTCCTTGCAACATAGA NGG 10 8 17 8 24 0 100 5 10
    240605185720_rgmg CTCCCTCTATGTTGCAAGGAACCATGGTCTAG(+CAGTGT)GTACCAAGGGCCCTCCTTGCCCAACCACCAC NGG 10 8 17 8 24 0 100 5 10