Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185520_7ran GACAGTCCATCCATCCTTAACTGCAGAATCTG(+CAGTGT)GATCCCCTGCCCTCCAGTTTTGCTGCCGTTT NGG 10 8 17 8 24 0 100 5 10
    240605185518_441v TTGATAAACGGCAGCAAAACTGGAGGGCAGGG(+CAGTGT)GATCCAGATTCTGCAGTTAAGGATGGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605185516_zq8d ATATGTGTGTGTGTGTGTGTGTGTGTATATAG(+CAGTGT)GTACCTACAATCTTTGTGGAATAGATTCTAA NGG 10 8 17 8 24 0 100 5 10
    240605185515_qhcm TATGTTTAGAATCTATTCCACAAAGATTGTAG(+CAGTGT)GTACCTATATACACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240605185513_fcw8 GGAAGAGACCATCAAACTCATTGGGACTGGAG(+CAGTGT)GATCCTCAATGCTCTGCAAGAGCAGCCCATA NGG 10 8 17 8 24 0 100 5 10
    240605185511_mysi GGTGTTATGGGCTGCTCTTGCAGAGCATTGAG(+CAGTGT)GATCCTCCAGTCCCAATGAGTTTGATGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605185510_ozqd CTGCCTTGTGAAAAAATTTTCATGCCAAGTTG(+CAGTGT)GTACCTCTGAGAATAATTGGTGACTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605185509_rewn ATACACCTTTTAGTCACCAATTATTCTCAGAG(+CAGTGT)GTACCAACTTGGCATGAAAATTTTTTCACAA NGG 10 8 17 8 24 0 100 5 10
    240605185507_c445 TGACTTGCCAGAAATCGTGTTAGTCAGATGTG(+CAGTGT)GTACCACATGCCCATAACCCGAAAACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605185506_t9h1 TATCTCTCAAGTTTTCGGGTTATGGGCATGTG(+CAGTGT)GTACCACATCTGACTAACACGATTTCTGGCA NGG 10 8 17 8 24 0 100 5 10