Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185430_avws ACCAGGATGAAAGAAAGCAGGGCTAGGGCCAG(+CAGTGT)GTACCCAAGGGACCACCAGGATGCGTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605185428_lugz ACCCTTTCTCACGCATCCTGGTGGTCCCTTGG(+CAGTGT)GTACCTGGCCCTAGCCCTGCTTTCTTTCATC NGG 10 8 17 8 24 0 100 5 10
    240605185427_vhfk CCAGAACTGAGCACTGCCGAAATGTTGGATTG(+CAGTGT)GATCCAGGTCTTGACTGACTCTCAGCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605185425_pqd5 GGTGGCCTTTGCTGAGAGTCAGTCAAGACCTG(+CAGTGT)GATCCAATCCAACATTTCGGCAGTGCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605185424_1o2d GGTCCCTTTCCCCGTTGGTCTACCTCCTTCTG(+CAGTGT)GATCCCACATACCCAGGGTCAAGCCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605185422_bgfb CAGGGACCCAGGGCTTGACCCTGGGTATGTGG(+CAGTGT)GATCCAGAAGGAGGTAGACCAACGGGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605185420_j6tr ACATGTATATCTCTACACCATGATTGGGCCTG(+CAGTGT)GTACCCAAAGGAGGACAGATGAGAGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605185419_vobw GGGCTCTGATTCTCTCATCTGTCCTCCTTTGG(+CAGTGT)GTACCAGGCCCAATCATGGTGTAGAGATATA NGG 10 8 17 8 24 0 100 5 10
    240605185417_pokq ACTGAAAGGTTTAAGAGGAAATGGGGTGATTG(+CAGTGT)GTACCAAGCAGCAGGAGGTAGACTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605185416_ac5g GTAGCAAACTGAAGTCTACCTCCTGCTGCTTG(+CAGTGT)GTACCAATCACCCCATTTCCTCTTAAACCTT NGG 10 8 17 8 24 0 100 5 10