Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185313_qd2b TAGTCCCAGGATTGGGGAGGTAGAGACAGGAG(+CAGTGT)GATCCAGAGTTCATGGTCCTCAGCTACATAG NGG 10 8 17 8 24 0 100 5 10
    240605185312_qqiu GTTTCCTATGTAGCTGAGGACCATGAACTCTG(+CAGTGT)GATCCTCCTGTCTCTACCTCCCCAATCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605185310_o2nt TACTGGCCTGAGGTGACAGGGTGGGAGAGCTG(+CAGTGT)GATCCATGAGGGAGCATGGTAGCCCGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605185309_7gg4 AGGTGGCTTTCGGGCTACCATGCTCCCTCATG(+CAGTGT)GATCCAGCTCTCCCACCCTGTCACCTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605185308_o07v CTCTCTCCAGTTAGTACCTATCTGGGATATTG(+CAGTGT)GTACCTTTGGATATGAATGTCCTTATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605185306_7aj3 ATATTTTCTTATAAGGACATTCATATCCAAAG(+CAGTGT)GTACCAATATCCCAGATAGGTACTAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240605185304_q7ag TGAAACTGGACTTGCTGGGGTGTCGCTAAGAG(+CAGTGT)GATCCCATTTGACCTCTGCCTTGCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240605185303_3f4y TCTCAGGCTGTGGCAAGGCAGAGGTCAAATGG(+CAGTGT)GATCCTCTTAGCGACACCCCAGCAAGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605185301_ejai CTTTGCTGCCTAAGAACCGGAGCCAACAGCAG(+CAGTGT)GTACCTCCTGGGGTTCCCTGAGGAGTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605185300_s622 GTTTTAGCACACTCCTCAGGGAACCCCAGGAG(+CAGTGT)GTACCTGCTGTTGGCTCCGGTTCTTAGGCAG NGG 10 8 17 8 24 0 100 5 10