Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190052_jnoo CTTATGAGCAAGCACCCACATTCCTAGCTGGG(+CAGTGT)GATCCCACGCTTCTGTGCCTCACTCCCACCC NGG 10 8 17 8 24 0 100 5 10
    240605190050_84pe TCACACAACCATTTATAACTCTGGTTCCAGGG(+CAGTGT)GATCCCACACCCTATTCTGACCTCTACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605190048_hcrs TCAGTGCCTGTAGAGGTCAGAATAGGGTGTGG(+CAGTGT)GATCCCCTGGAACCAGAGTTATAAATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605190046_s6j3 TAGATCAGGCTCTCTAGCTAGGAAGCCCTTGG(+CAGTGT)GATCCTCCTGACTCTTAAGCACTGAGATTAC NGG 10 8 17 8 24 0 100 5 10
    240605190044_66uj CACTTGTAATCTCAGTGCTTAAGAGTCAGGAG(+CAGTGT)GATCCCAAGGGCTTCCTAGCTAGAGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605190042_dgrs CCCTCAGCCCTCAACCCCTGCTCACCCCTCAG(+CAGTGT)GATCCTGCTGCACTCAGCTTCCGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240605190040_yy8x GCTGCTGTTCCTGCGGAAGCTGAGTGCAGCAG(+CAGTGT)GATCCTGAGGGGTGAGCAGGGGTTGAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605190039_3hon CTGCTGTCCCGACTCCTGCACAGGCTGAGGTG(+CAGTGT)GTACCAAGATGGCCATCTGTGGCTACTCACC NGG 10 8 17 8 24 0 100 5 10
    240605190038_06wv TTGCCGGTGAGTAGCCACAGATGGCCATCTTG(+CAGTGT)GTACCACCTCAGCCTGTGCAGGAGTCGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605190035_fr5x AGGGGACGGCCAGACAGTACAGTCACCCATAG(+CAGTGT)GATCCCCAGAGACATAAAGGGTATCAGGACA NGG 10 8 17 8 24 0 100 5 10