Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185332_aj6o AACATGAAATTGAGTAGTGTGCCAATAACTTG(+CAGTGT)GATCCTGCCACTGTAGTCTGTCTTTGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605185330_2tf2 AAGCAACTTCCAAAGACAGACTACAGTGGCAG(+CAGTGT)GATCCAAGTTATTGGCACACTACTCAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605185328_bpb1 AGAACGAGAACAGACACGTTGCAGGTAAGAGG(+CAGTGT)GATCCAGTGGGAGAGATTCACAGAAAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605185326_w589 GTGCACCACCTTTCTGTGAATCTCTCCCACTG(+CAGTGT)GATCCCTCTTACCTGCAACGTGTCTGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605185324_nbcd TACTTGAGCACCACACAAAGGCTGGGATAAGG(+CAGTGT)GTACCTTCTGATGTTTTCTCAGAGCTGGGCG NGG 10 8 17 8 24 0 100 5 10
    240605185323_cmz6 TTTCCCGCCCAGCTCTGAGAAAACATCAGAAG(+CAGTGT)GTACCCTTATCCCAGCCTTTGTGTGGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605185321_9v87 GCCCCAGTCACTTCCCACTCCCTGCTCTCCAG(+CAGTGT)GTACCACCCGGGTCAGTGAGATACTTGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605185319_qdqz AGACCATGCCAAGTATCTCACTGACCCGGGTG(+CAGTGT)GTACCTGGAGAGCAGGGAGTGGGAAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240605185317_9f7z TCTCACAACCACCTACAACTCCAATTCCAAGG(+CAGTGT)GATCCAATGGCATCTTCTGGCTTCTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605185315_sz6q CCTGGTCCCTCAGAAGCCAGAAGATGCCATTG(+CAGTGT)GATCCCTTGGAATTGGAGTTGTAGGTGGTTG NGG 10 8 17 8 24 0 100 5 10