Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185446_d211 CAGGCGGGCCTCTTTCCTCTCATTTAACCCAG(+CAGTGT)GATCCTTAGGCTGAAACAGTTCCCCAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605185444_4hvw CTGCTCTGCTTGGGGAACTGTTTCAGCCTAAG(+CAGTGT)GATCCTGGGTTAAATGAGAGGAAAGAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605185443_lis4 AAGAGAGCTAGGGGTGGGACAGTCCAAACTGG(+CAGTGT)GTACCCATAGCCCAGTCTCGGTCAAGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605185441_p6lj AGGGGACCAGCTTGACCGAGACTGGGCTATGG(+CAGTGT)GTACCCAGTTTGGACTGTCCCACCCCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605185440_cnub CTGGCTTGTAGCGCCCCCTGTCTTGACACTCG(+CAGTGT)GATCCCCAATTGCTCCTCTGTTTTCCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605185438_zld3 GGCCAAAAAGGGAAAACAGAGGAGCAATTGGG(+CAGTGT)GATCCGAGTGTCAAGACAGGGGGCGCTACAA NGG 10 8 17 8 24 0 100 5 10
    240605185437_3ip5 CTGGGTAGTAGTTGAATAACAAAATGTTTACG(+CAGTGT)GATCCAATTAGATTCATGGTACTCCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605185436_eiwg CTCATGACTCTGGAGTACCATGAATCTAATTG(+CAGTGT)GATCCGTAAACATTTTGTTATTCAACTACTA NGG 10 8 17 8 24 0 100 5 10
    240605185434_n6ht GGATGCCGAGAAAACGCTGAGAGACCATCTCG(+CAGTGT)GATCCTAGGGGAGGTTCCATTTATTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605185432_04mf GCTTCCCTTCAAATAAATGGAACCTCCCCTAG(+CAGTGT)GATCCGAGATGGTCTCTCAGCGTTTTCTCGG NGG 10 8 17 8 24 0 100 5 10