Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185923_xdqb CTGAAAATATGAAGAATTATTTTTATACAAAG(+CAGTGT)GATCCTTAAGCTTGGAGCACAATAAAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605185921_2wou AGAGGTCATCTTTATTGTGCTCCAAGCTTAAG(+CAGTGT)GATCCTTTGTATAAAAATAATTCTTCATATT NGG 10 8 17 8 24 0 100 5 10
    240605185920_vgeq AAGCACTGAGTGCCATTAGCTAAATGCATAGG(+CAGTGT)GTACCACCCACAGGTGCCAGGGGCCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605185918_p8af ACTTTGGGAAAGGCCCCTGGCACCTGTGGGTG(+CAGTGT)GTACCCTATGCATTTAGCTAATGGCACTCAG NGG 10 8 17 8 24 0 100 5 10
    240605185916_n2vh TTTGGATCTTTAGTCAAATTGGCTAAGACTTG(+CAGTGT)GATCCCAGAACCCAAGAAGAAAGGAAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605185915_nj17 TTGGGTCCCCTTCCTTTCTTCTTGGGTTCTGG(+CAGTGT)GATCCAAGTCTTAGCCAATTTGACTAAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605185913_7mx6 CCATCTTGTCTCTTCCCTACTGTCTTCAGTGG(+CAGTGT)GATCCCCTCTTGGGTCCCTCCCTTTGTCATC NGG 10 8 17 8 24 0 100 5 10
    240605185911_yupz CACATGATGACAAAGGGAGGGACCCAAGAGGG(+CAGTGT)GATCCCACTGAAGACAGTAGGGAAGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240605185909_2p23 GTAGGTCGAGCTAGAGGGCTACAGAGAGGCAG(+CAGTGT)GATCCAGGATTAGAGAAATTCCTAAGATCTG NGG 10 8 17 8 24 0 100 5 10
    240605185908_dcaw TCTGGCAGATCTTAGGAATTTCTCTAATCCTG(+CAGTGT)GATCCTGCCTCTCTGTAGCCCTCTAGCTCGA NGG 10 8 17 8 24 0 100 5 10