Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190156_9jp2 CATCTGTCTTTCTACTCCTTCAGTTGTATGGG(+CAGTGT)GATCCAGGGATCTGTTAAATATTAGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605190153_1fr5 TCCATACTTCCCTAATATTTAACAGATCCCTG(+CAGTGT)GATCCCCATACAACTGAAGGAGTAGAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605190152_am5c GCTCCACTCCATGTGAGAGCTTTCCTTGGCAG(+CAGTGT)GTACCCCATGGCTCTGGCAGCCCCAAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605190150_eaov CCCCAAGAGCTTGGGGCTGCCAGAGCCATGGG(+CAGTGT)GTACCTGCCAAGGAAAGCTCTCACATGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605190149_dcu1 ATCTGGAGAAATAGTTCAGAGTTCTACATCTG(+CAGTGT)GATCCACAGGCAGCAGGAAGAGAGAGCCATT NGG 10 8 17 8 24 0 100 5 10
    240605190147_f4kr GACCCAATGGCTCTCTCTTCCTGCTGCCTGTG(+CAGTGT)GATCCAGATGTAGAACTCTGAACTATTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605190146_30l5 AGCAGACCGTCTCGGGACTGGAACCCCCGAAG(+CAGTGT)GATCCTTGCCCCACAGCCTGTCGTCCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240605190145_nqkr CAACACCCGAGGACGACAGGCTGTGGGGCAAG(+CAGTGT)GATCCTTCGGGGGTTCCAGTCCCGAGACGGT NGG 10 8 17 8 24 0 100 5 10
    240605190143_0n4d GTGCACTCATGCACACAGGCATAGAGGGCCTG(+CAGTGT)GATCCTACCTGCTCATCTGGTGTCAAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605190141_wkw6 TGTTCGAAGCTTGACACCAGATGAGCAGGTAG(+CAGTGT)GATCCAGGCCCTCTATGCCTGTGTGCATGAG NGG 10 8 17 8 24 0 100 5 10